Comparative Evaluation of a Novel Recombinase Polymerase Amplification-Lateral Flow Dipstick (RPA-LFD) Assay, LAMP, Conventional PCR, and Leaf-Disc Baiting Methods for Detection of Phytophthora sojae.
Identifieur interne : 000557 ( Main/Exploration ); précédent : 000556; suivant : 000558Comparative Evaluation of a Novel Recombinase Polymerase Amplification-Lateral Flow Dipstick (RPA-LFD) Assay, LAMP, Conventional PCR, and Leaf-Disc Baiting Methods for Detection of Phytophthora sojae.
Auteurs : Tingting Dai [République populaire de Chine] ; Xiao Yang [États-Unis] ; Tao Hu [République populaire de Chine] ; Binbin Jiao [République populaire de Chine] ; Yue Xu [République populaire de Chine] ; Xiaobo Zheng [République populaire de Chine] ; Danyu Shen [République populaire de Chine]Source :
- Frontiers in microbiology [ 1664-302X ] ; 2019.
Abstract
Early and accurate detection of the causal pathogen Phytophthora sojae is crucial for effective prevention and control of root and stem rot and seedling damping-off of soybean. In the present study, a novel isothermal amplification assay was developed for detecting P. sojae. This 25 min assay included a two-step approach. First, a pair of novel primers, PSYPT-F and PSYPT-R were used to amplify a specific fragment of the Ypt1 gene of P. sojae in a 20 min recombinase polymerase amplification (RPA) step. Second, lateral flow dipsticks (LFD) were used to detect and visualize RPA amplicons of P. sojae within 5 min. This RPA-LFD assay was specific to P. sojae. It yielded negative detection results against 24 other Phytophthora, one Globisporangium, and 14 fungal species. It was also found to be sensitive, detecting as low as 10 pg of P. sojae genomic DNA in a 50-μL reaction. Furthermore, P. sojae was detected from artificially inoculated hypocotyls of soybean seedlings using this novel assay. In a comparative evaluation using 130 soybean rhizosphere samples, this novel assay consistently detected P. sojae in 55.4% of samples, higher than other three methods, including loop-mediated isothermal amplification (54.6%), conventional PCR (46.9%), and leaf-disc baiting (38.5-40.0%). Results in this study indicated that this rapid, specific, and sensitive RPA-LFD assay has potentially significant applications to diagnosing Phytophthora root and stem rot and damp-off of soybean, especially under time- and resource-limited conditions.
DOI: 10.3389/fmicb.2019.01884
PubMed: 31447827
PubMed Central: PMC6696978
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparative Evaluation of a Novel Recombinase Polymerase Amplification-Lateral Flow Dipstick (RPA-LFD) Assay, LAMP, Conventional PCR, and Leaf-Disc Baiting Methods for Detection of <i>Phytophthora sojae</i>.</title><author><name sortKey="Dai, Tingting" sort="Dai, Tingting" uniqKey="Dai T" first="Tingting" last="Dai">Tingting Dai</name><affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Xiao" sort="Yang, Xiao" uniqKey="Yang X" first="Xiao" last="Yang">Xiao Yang</name><affiliation wicri:level="2"><nlm:affiliation>Foreign Disease-Weed Science Research Unit, USDA, Agricultural Research Service, Fort Detrick, MD, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Foreign Disease-Weed Science Research Unit, USDA, Agricultural Research Service, Fort Detrick, MD</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>ARS Research Participation Program, Oak Ridge Institute for Science and Education, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>ARS Research Participation Program, Oak Ridge Institute for Science and Education, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Hu, Tao" sort="Hu, Tao" uniqKey="Hu T" first="Tao" last="Hu">Tao Hu</name><affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Jiao, Binbin" sort="Jiao, Binbin" uniqKey="Jiao B" first="Binbin" last="Jiao">Binbin Jiao</name><affiliation wicri:level="1"><nlm:affiliation>Technical Center for Animal, Plant and Food Inspection and Quarantine of Shanghai Customs, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Technical Center for Animal, Plant and Food Inspection and Quarantine of Shanghai Customs, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Yue" sort="Xu, Yue" uniqKey="Xu Y" first="Yue" last="Xu">Yue Xu</name><affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Xiaobo" sort="Zheng, Xiaobo" uniqKey="Zheng X" first="Xiaobo" last="Zheng">Xiaobo Zheng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Shen, Danyu" sort="Shen, Danyu" uniqKey="Shen D" first="Danyu" last="Shen">Danyu Shen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31447827</idno><idno type="pmid">31447827</idno><idno type="doi">10.3389/fmicb.2019.01884</idno><idno type="pmc">PMC6696978</idno><idno type="wicri:Area/Main/Corpus">000399</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000399</idno><idno type="wicri:Area/Main/Curation">000399</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000399</idno><idno type="wicri:Area/Main/Exploration">000399</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Comparative Evaluation of a Novel Recombinase Polymerase Amplification-Lateral Flow Dipstick (RPA-LFD) Assay, LAMP, Conventional PCR, and Leaf-Disc Baiting Methods for Detection of <i>Phytophthora sojae</i>.</title><author><name sortKey="Dai, Tingting" sort="Dai, Tingting" uniqKey="Dai T" first="Tingting" last="Dai">Tingting Dai</name><affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Xiao" sort="Yang, Xiao" uniqKey="Yang X" first="Xiao" last="Yang">Xiao Yang</name><affiliation wicri:level="2"><nlm:affiliation>Foreign Disease-Weed Science Research Unit, USDA, Agricultural Research Service, Fort Detrick, MD, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Foreign Disease-Weed Science Research Unit, USDA, Agricultural Research Service, Fort Detrick, MD</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation><affiliation wicri:level="2"><nlm:affiliation>ARS Research Participation Program, Oak Ridge Institute for Science and Education, Oak Ridge, TN, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>ARS Research Participation Program, Oak Ridge Institute for Science and Education, Oak Ridge, TN</wicri:regionArea><placeName><region type="state">Tennessee</region></placeName></affiliation></author><author><name sortKey="Hu, Tao" sort="Hu, Tao" uniqKey="Hu T" first="Tao" last="Hu">Tao Hu</name><affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Jiao, Binbin" sort="Jiao, Binbin" uniqKey="Jiao B" first="Binbin" last="Jiao">Binbin Jiao</name><affiliation wicri:level="1"><nlm:affiliation>Technical Center for Animal, Plant and Food Inspection and Quarantine of Shanghai Customs, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Technical Center for Animal, Plant and Food Inspection and Quarantine of Shanghai Customs, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Yue" sort="Xu, Yue" uniqKey="Xu Y" first="Yue" last="Xu">Yue Xu</name><affiliation wicri:level="1"><nlm:affiliation>College of Forestry, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Forestry, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Xiaobo" sort="Zheng, Xiaobo" uniqKey="Zheng X" first="Xiaobo" last="Zheng">Xiaobo Zheng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Shen, Danyu" sort="Shen, Danyu" uniqKey="Shen D" first="Danyu" last="Shen">Danyu Shen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Plant Pathology, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in microbiology</title><idno type="ISSN">1664-302X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Early and accurate detection of the causal pathogen <i>Phytophthora sojae</i> is crucial for effective prevention and control of root and stem rot and seedling damping-off of soybean. In the present study, a novel isothermal amplification assay was developed for detecting <i>P. sojae</i>. This 25 min assay included a two-step approach. First, a pair of novel primers, PSYPT-F and PSYPT-R were used to amplify a specific fragment of the <i>Ypt1</i> gene of <i>P. sojae</i> in a 20 min recombinase polymerase amplification (RPA) step. Second, lateral flow dipsticks (LFD) were used to detect and visualize RPA amplicons of <i>P. sojae</i> within 5 min. This RPA-LFD assay was specific to <i>P. sojae</i>. It yielded negative detection results against 24 other <i>Phytophthora</i>, one <i>Globisporangium</i>, and 14 fungal species. It was also found to be sensitive, detecting as low as 10 pg of <i>P. sojae</i> genomic DNA in a 50-μL reaction. Furthermore, <i>P. sojae</i> was detected from artificially inoculated hypocotyls of soybean seedlings using this novel assay. In a comparative evaluation using 130 soybean rhizosphere samples, this novel assay consistently detected <i>P. sojae</i> in 55.4% of samples, higher than other three methods, including loop-mediated isothermal amplification (54.6%), conventional PCR (46.9%), and leaf-disc baiting (38.5-40.0%). Results in this study indicated that this rapid, specific, and sensitive RPA-LFD assay has potentially significant applications to diagnosing Phytophthora root and stem rot and damp-off of soybean, especially under time- and resource-limited conditions.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">31447827</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>29</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-302X</ISSN><JournalIssue CitedMedium="Print"><Volume>10</Volume><PubDate><Year>2019</Year></PubDate></JournalIssue><Title>Frontiers in microbiology</Title><ISOAbbreviation>Front Microbiol</ISOAbbreviation></Journal><ArticleTitle>Comparative Evaluation of a Novel Recombinase Polymerase Amplification-Lateral Flow Dipstick (RPA-LFD) Assay, LAMP, Conventional PCR, and Leaf-Disc Baiting Methods for Detection of <i>Phytophthora sojae</i>.</ArticleTitle><Pagination><MedlinePgn>1884</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fmicb.2019.01884</ELocationID><Abstract><AbstractText>Early and accurate detection of the causal pathogen <i>Phytophthora sojae</i> is crucial for effective prevention and control of root and stem rot and seedling damping-off of soybean. In the present study, a novel isothermal amplification assay was developed for detecting <i>P. sojae</i>. This 25 min assay included a two-step approach. First, a pair of novel primers, PSYPT-F and PSYPT-R were used to amplify a specific fragment of the <i>Ypt1</i> gene of <i>P. sojae</i> in a 20 min recombinase polymerase amplification (RPA) step. Second, lateral flow dipsticks (LFD) were used to detect and visualize RPA amplicons of <i>P. sojae</i> within 5 min. This RPA-LFD assay was specific to <i>P. sojae</i>. It yielded negative detection results against 24 other <i>Phytophthora</i>, one <i>Globisporangium</i>, and 14 fungal species. It was also found to be sensitive, detecting as low as 10 pg of <i>P. sojae</i> genomic DNA in a 50-μL reaction. Furthermore, <i>P. sojae</i> was detected from artificially inoculated hypocotyls of soybean seedlings using this novel assay. In a comparative evaluation using 130 soybean rhizosphere samples, this novel assay consistently detected <i>P. sojae</i> in 55.4% of samples, higher than other three methods, including loop-mediated isothermal amplification (54.6%), conventional PCR (46.9%), and leaf-disc baiting (38.5-40.0%). Results in this study indicated that this rapid, specific, and sensitive RPA-LFD assay has potentially significant applications to diagnosing Phytophthora root and stem rot and damp-off of soybean, especially under time- and resource-limited conditions.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dai</LastName><ForeName>Tingting</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>College of Forestry, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Xiao</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Foreign Disease-Weed Science Research Unit, USDA, Agricultural Research Service, Fort Detrick, MD, United States.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>ARS Research Participation Program, Oak Ridge Institute for Science and Education, Oak Ridge, TN, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Tao</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>College of Forestry, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiao</LastName><ForeName>Binbin</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Technical Center for Animal, Plant and Food Inspection and Quarantine of Shanghai Customs, Shanghai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Yue</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Forestry, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Xiaobo</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>Danyu</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Nanjing Agricultural University, Nanjing, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>08</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Microbiol</MedlineTA><NlmUniqueID>101548977</NlmUniqueID><ISSNLinking>1664-302X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Phytophthora melonis</Keyword><Keyword MajorTopicYN="N">Phytophthora sansomeana</Keyword><Keyword MajorTopicYN="N">Phytophthora vignae</Keyword><Keyword MajorTopicYN="N">field diagnosis</Keyword><Keyword MajorTopicYN="N">oomycetes</Keyword><Keyword MajorTopicYN="N">plant destroyers</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>05</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>07</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>8</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>8</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>8</Month><Day>27</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31447827</ArticleId><ArticleId IdType="doi">10.3389/fmicb.2019.01884</ArticleId><ArticleId IdType="pmc">PMC6696978</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Fungal Genet Biol. 2000 Jun;30(1):17-32</Citation><ArticleIdList><ArticleId IdType="pubmed">10955905</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2007 Nov 1;23(21):2947-8</Citation><ArticleIdList><ArticleId IdType="pubmed">17846036</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2006 Dec;96(12):1315-21</Citation><ArticleIdList><ArticleId IdType="pubmed">18943663</ArticleId></ArticleIdList></Reference><Reference><Citation>J Nematol. 2006 Jun;38(2):173-80</Citation><ArticleIdList><ArticleId IdType="pubmed">19259444</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycologia. 2009 Jan-Feb;101(1):129-35</Citation><ArticleIdList><ArticleId IdType="pubmed">19271675</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2007 Jan;8(1):1-8</Citation><ArticleIdList><ArticleId IdType="pubmed">20507474</ArticleId></ArticleIdList></Reference><Reference><Citation>Fungal Biol. 2011 Aug;115(8):733-40</Citation><ArticleIdList><ArticleId IdType="pubmed">21802053</ArticleId></ArticleIdList></Reference><Reference><Citation>FEMS Microbiol Lett. 2012 Sep;334(1):27-34</Citation><ArticleIdList><ArticleId IdType="pubmed">22697582</ArticleId></ArticleIdList></Reference><Reference><Citation>Parasit Vectors. 2015 Sep 04;8:446</Citation><ArticleIdList><ArticleId IdType="pubmed">26338510</ArticleId></ArticleIdList></Reference><Reference><Citation>Expert Rev Mol Diagn. 2015;15(11):1475-89</Citation><ArticleIdList><ArticleId IdType="pubmed">26517245</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Chem. 2016 Jul;62(7):947-58</Citation><ArticleIdList><ArticleId IdType="pubmed">27160000</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2017 Jan 09;7:40244</Citation><ArticleIdList><ArticleId IdType="pubmed">28067278</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2017 Apr 25;12(4):e0176567</Citation><ArticleIdList><ArticleId IdType="pubmed">28441441</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2018 Jan 4;46(D1):D41-D47</Citation><ArticleIdList><ArticleId IdType="pubmed">29140468</ArticleId></ArticleIdList></Reference><Reference><Citation>IMA Fungus. 2017 Dec;8(2):355-384</Citation><ArticleIdList><ArticleId IdType="pubmed">29242780</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2018 Oct 29;8(1):15972</Citation><ArticleIdList><ArticleId IdType="pubmed">30374117</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2017 Jul;101(7):1171-1181</Citation><ArticleIdList><ArticleId IdType="pubmed">30682964</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2010 Jul;94(7):881-884</Citation><ArticleIdList><ArticleId IdType="pubmed">30743553</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2004 Oct;88(10):1139-1145</Citation><ArticleIdList><ArticleId IdType="pubmed">30795257</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 1993 Aug 25;21(17):4153-4</Citation><ArticleIdList><ArticleId IdType="pubmed">8371994</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Maryland</li><li>Tennessee</li></region></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Dai, Tingting" sort="Dai, Tingting" uniqKey="Dai T" first="Tingting" last="Dai">Tingting Dai</name></noRegion><name sortKey="Hu, Tao" sort="Hu, Tao" uniqKey="Hu T" first="Tao" last="Hu">Tao Hu</name><name sortKey="Jiao, Binbin" sort="Jiao, Binbin" uniqKey="Jiao B" first="Binbin" last="Jiao">Binbin Jiao</name><name sortKey="Shen, Danyu" sort="Shen, Danyu" uniqKey="Shen D" first="Danyu" last="Shen">Danyu Shen</name><name sortKey="Xu, Yue" sort="Xu, Yue" uniqKey="Xu Y" first="Yue" last="Xu">Yue Xu</name><name sortKey="Zheng, Xiaobo" sort="Zheng, Xiaobo" uniqKey="Zheng X" first="Xiaobo" last="Zheng">Xiaobo Zheng</name></country><country name="États-Unis"><region name="Maryland"><name sortKey="Yang, Xiao" sort="Yang, Xiao" uniqKey="Yang X" first="Xiao" last="Yang">Xiao Yang</name></region><name sortKey="Yang, Xiao" sort="Yang, Xiao" uniqKey="Yang X" first="Xiao" last="Yang">Xiao Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000557 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000557 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:31447827
|texte= Comparative Evaluation of a Novel Recombinase Polymerase Amplification-Lateral Flow Dipstick (RPA-LFD) Assay, LAMP, Conventional PCR, and Leaf-Disc Baiting Methods for Detection of Phytophthora sojae.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31447827" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |